Molecular and epidemiological characterization of human adenovirus and classic human astrovirus in children with acute diarrhea in Shanghai, 2017–2018

Abstract Background In addition to rotavirus and norovirus, human adenovirus (HAdV) classic astrovirus (classic HAstV) are important pathogens of acute diarrhea in infants young children. Here, we present the molecular epidemiology HAdV HAstV children with Shanghai. Methods Fecal specimens were collected from 804 outpatient diagnosed Shanghai January 2017 December 2018. All samples screened for presence HAstV. detected using traditional PCR reverse-transcription PCR, respectively. positive genotyped by phylogenetic analysis. Results Among fecal samples, 8.58% (69/804) infected either or HAstV, five co-infected two viruses. The overall detection rates 3.47% (28/804) 5.22% (42/804), Four subgroups (A, B, C, F) seven genotypes (HAdV-C1, −C2, −B3, −C5, −A31, −F40, -F41) detected. Subgroup F had highest constituent ratio at 64.29% (18/28), followed non-enteric subgroup C (21.43%, 6/28) B 10.71% (3/28). HAdV-F41 (60.71%, 17/28) was dominant genotype, HAdV-C2 (14.29%, 4/28) HAdV-B3 (10.71%, 3/28). Two (HAstV-1 HAstV-5) identified 42 during study period; HAstV-1 (95.24%, 40/42) predominant other strains as HAstV-5. No significant differences found between boys girls ( P = 0.604) 0.275). Over half infections (82.14%, 23/28) (66.67%, 28/42) occurred less than 36 months. Seasonal preferences summer winter, this study, common clinical symptoms diarrhea, vomiting, fever abdominal pain. Conclusions Our findings indicate that play roles pathogenesis Systematic long-term surveillance needed monitor their prevalence prevent major outbreak.